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KMID : 1098420060140010023
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 1 p.23 ~ p.26
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Micropropagation of Hypericum erectum by Axillary Bud Culture
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Kim Mei-Lan
Nam Dong-Woo
Ahn Jun-Cheul
Hwang Baik
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Abstract
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One of micropropagation methods was investigated by using a multiple-shoots protocol. Multiple shoot formation was obtained from excised axillary buds of Hypericum erectum on half-strength or basal MS medium supplemented with TDZ or BA. The optimal combination of shoot multiplication for the production of more shoots with a suitable size was MS medium supplemented with 0.005;mg{cdot}L^{-1} TDZ (6.5 adventitious shoots per node). In vitro rooting was carried on half-strength MS medium with 1;mg{cdot}L^{-1};GA_3;and;0.5;mg{cdot}L^{-1} IBA treatment. In addition, the rooted cuttings were showed a better root growth in the greenhouse and survived in more than 90%. The results show that the species can be micropropagated effectively by the application of axillary bud culture systems.
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KEYWORD
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micropropagation, shoot multiplication, in vitro rooting
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